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Adam Siepel Seminar

February 27 @ 4:00 pm - 5:00 pm

Seminar Flyer - Adam Siepel copy

Abstract:  Transcriptional regulatory changes have been shown to contribute to phenotypic differences between species, but many questions remain about how gene expression evolves. In this talk, I will present the first comparative study of nascent transcription in primates. We used PRO-seq to map actively transcribing RNA polymerases in resting and activated CD4+ T-cells in multiple human, chimpanzee, and rhesus macaque individuals, with rodents as outgroups. This approach allowed us to directly measure active transcription separately from post-transcriptional processes. We observed general conservation in coding and non-coding transcription, punctuated by numerous differences between species, particularly at distal enhancers and non-coding RNAs. Transcription factor binding sites are a primary determinant of transcriptional differences between species. We found evidence for stabilizing selection on gene expression levels and adaptive substitutions associated with lineage-specific transcription. Finally, rates of evolutionary change are strongly correlated with long-range chromatin interactions. These observations clarify the role of primary transcription in regulatory evolution.

Details

Date:
February 27
Time:
4:00 pm - 5:00 pm

Venue

Boyer Hall 159

Upcoming Events

  1. Markus Covert Seminar

    April 3 @ 4:00 pm - 5:00 pm
  2. Brandon Gaut Seminar

    April 10 @ 4:00 pm - 5:00 pm
  3. Zeba Wunderlich Seminar

    April 17 @ 4:00 pm - 5:00 pm
  4. Jun Song Seminar

    April 24 @ 4:00 pm - 5:00 pm
  5. 2nd Annual QCBio Symposium

    April 28 @ 8:30 am - 4:00 pm

Recent Student Publications

Determining the Effect of Natural Selection on Linked Neutral Divergence across Species
Phung TN, Huber CD, Lohmueller KE.
PLOS Genetics. August 2016.

PReFerSim: fast simulation of demography and selection under the Poisson Random Field model
Ortega-Del Vecchyo D, Mardsen CD, Lohmueller KE.
Bioinformatics. July 2016.

SURVIV for survival analysis of mRNA isoform variation
Shen S, Wang Y, Wang C, Wu YN, Xing Y.
Nature Communications. June 2016.